anti gfrα1  (R&D Systems)

Journal: Frontiers in Neurology

Article Title: KCNB1 mutation impairs neuronal differentiation by disrupting gene expression temporal regulation and neuron-specific pathways

doi: 10.3389/fneur.2026.1739214

Figure Lengend Snippet: Induced differentiation of iPSCs. (A) Experimental schematic of the iPSC differentiation process. (B) Representative morphological images of healthy donor-derived NPCs at neuron day 5 and forebrain precursors at neuron day 10. (C) Representative images of immunofluorescence-stained neurons derived from the healthy donor. Neurons were stained with MAP2 (green) and NeuN (red). Nuclei were stained with DAPI (blue). Scale bar: 10 μm. (D) Representative morphological images of patient-derived NPCs at neuron day 5 and forebrain precursors at neuron day 10. (E) Representative images of immunofluorescence-stained neurons derived from the patient. Neurons were stained with MAP2 (green) and NeuN (red). Nuclei were stained with DAPI (blue). Scale bar: 10 μm. BDNF, Brain-Derived Neurotrophic Factor; GDNF, Glial Cell Line-Derived Neurotrophic Factor; LAA, L-Ascorbic acid.

Article Snippet: Recombinant Human GDNF Protein , Novoprotein , C226.

Techniques: Derivative Assay, Immunofluorescence, Staining